16S V3-V4 Library Preparation Kit

16S V3-V4 Library Preparation Kit for Metagenomics

Uncoded brings you it’s first ever product, the 16S V3-V4 Library Preparation Kit, for metagenomics. Metagenomics sequencing method enhances researcher’s understanding of bacterial diversity and phylogeny by targeting the 16S ribosomal RNA (rRNA) gene that offer detailed insights into microbial communities. Despite its promising potential, researchers often face challenges with complex protocols, high costs, data loss, compromised sequencing quality, and high limits of detection (LOD).

Addressing Challenges

These challenges are addressed with this kit as it targets the highly variable V3-V4 region of the 16S rRNA gene, providing robust solution to identify diverse microbial populations with difficult sample types (stool & soil). It utilizes phased primers and high-quality reagents offering a cost-effective, all-in-one solution. The libraries are prepared and sequenced using a user-friendly protocol. This kit has been tested and optimized on diverse sample types and wide range of bacterial load (low to high), ensuring reliable and precise results.

Research
Product Name
16S V3-V4 Library Preparation Kit
Cat. No. Rxns
UN16S/0507/024 24
UN16S/0507/096 96
UN16S/0507/384 384
Storage temperature: -25 °C to -15 °C
Ref No: UN16S/0507/096/100 16S V3-V4 Library Preparation kit (1 of 3)
Storage temperature: 2°C to 8°C
Ref No: UN16S/0507/096/200 16S V3-V4 Library Preparation kit (2 of 3)
Storage temperature: -25 °C to -15 °C
Ref No: UN16S/0507/096/300 16S V3-V4 Library Preparation kit (3 of 3)

Consistency

Ensures reliable, reproducible results across different samples without background noise by optimized protocol and implementing QMS guidelines

User-Friendly

Kits intuitive design, allowing for easy & straightforward workflow with minimal hands-ontime & reduce technical complexity

Scalability

Effectively supporting both small & large scale projects with consistent performance & adaptability across
various sample types for different Illumina platforms

Technical Support

Benefit from our dedicated technical support team from expert guidance and troubleshooting

Get Your Answers Here

  • What are the DNA quality Requirements?

    High-quality genomic DNA is essential for successful 16S metagenomics analysis. The DNA should be free from contaminants like heme, humic acid, polysaccharides, polyphenols, or any substances carried over from the sample source. Contaminants introduced during DNA extraction can impact the quality of the sequencing run, so it is important to ensure that the DNA is pure and clean to achieve reliable results.

  • How should I quantify the DNA samples?

    Use the Qubit (fluorescence-based method), as it provides more accurate quantification of DNA. Although Nanodrop (absorbance-based) can offer useful information on sample purity, it is not sufficient for accurate quantification.

  • How much DNA is needed for 16S V3- V4 Library preparation kit?

    The standard protocol for 16S V3- V4 Library preparation requires between 1-5 ng of total DNA (high quality). For this kit, 12.5 ng is recommended, and sufficient yields are attainable from as little as 100pg-50ng depending on the sample type.

  • What is the 16S V3- V4 Library preparation workflow?

    16S V3-V4 Library preparation workflow includes library preparation, sequencing on the Illumina® platform and data analysis.

  • What is the sensitivity of your 16S V3- V4 Library preparation assay?

    Our targeted 16S-library preparation kit typically provides sensitivity down to the genus level.

  • How does 16S V3- V4 Library preparation compare to other 16S methods on the market?

    The 16S V3-V4 Library Preparation offers several advantages over others, including industry-leading turnaround time. It is based on the phased primer concept to specifically target the highly variable V3 and V4 regions of the 16S rRNA gene, which enhances sensitivity and accuracy. The assay is cost-effective and has been validated across various sample types, ensuring reliable performance in diverse applications.

Learn more about 16S V3-V4

Download these resources for more information on the 16s V3-V4 Library Preparation Kit
Premas Life Sciences is proud and exhilarated to introduce Uncoded, an initiative that marks a significant milestone in our journey of scientific innovation.
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